Protocols

The HMBR includes protocols for microbiome bioactive discovery from early-stage computational prioritization through molecular and in vivo discovery and validation assays:

Sample collection and handling

Stool Kit Assembly Doc

Molecular Data Generation

Metabolomics HMP2 Protocol
Metagenomics HMP2 Protocol
Metatranscriptomics HMP2 Protocol
DNA and RNA co-extraction from stool

Mouse Protocols

Gnotobiotic Semi Rigid Isolators
Gnotobiotic Tecniplast IVC

Mammalian Cell Protocols

Murine bone marrow-derived dendritic cell (BMDC) Isolation
IL-10 screening protocol from bone marrow-derived dendritic cells