The HMBR includes protocols for microbiome bioactive discovery from early-stage computational prioritization through molecular and in vivo discovery and validation assays:
Sample collection and handling
HMP2 Version – Stool Kit Assembly Doc
2021 Version (Collection Core) – https://hcmph.sph.harvard.edu/hcmcc/
Molecular Data Generation
Metabolomics HMP2 Protocol
Metagenomics HMP2 Protocol
Metatranscriptomics 2021 Protocol
Metatranscriptomics HMP2 Protocol
DNA and RNA co-extraction from stool (2021)
DNA and RNA co-extraction from stool (HMP2)
Mouse Protocols
Gnotobiotic Semi Rigid Isolators
Gnotobiotic Tecniplast IVC
Mammalian Cell Protocols
Murine bone marrow-derived dendritic cell (BMDC) Isolation
IL-10 screening protocol from bone marrow-derived dendritic cells